EST Data In MaizeGDB
EST Research Tools | Discussion of EST Data for the General Public
To find out about EST clusters/contigs, see the Sequence Data In MaizeGDB page.
EST Research Tools
Simple EST Search: This search form allows you to just enter basic name info to quickly retrieve the desired EST. Use % as a wild card.
If you want to search by accession number, try the sequence search.
If you want to search by a descriptive term like dehydrogenase, also use the sequence search.
If you want to search by gene name, like bz1 or wx, try the locus search.
The above search provides an example of the type of search you can do; it returns a listing of ESTs from the 486 library of the Maize Gene Discovery Project ("std" is short for Stanford, in this case). Other interesting searches include p-std606% (the 606 library) and %606001A02% (a specific member of the 606 library).
This search can be executed on every page on the site by utilizing the form in the upper right corner. Choose "EST" from the drop-down menu, enter your search term in the field, and hit return to find the ESTs you're looking for.
Mapped ESTs: This utility displays the map locations of the ESTs in the database. You can obtain this information by chromosome (1-10, below) or all together (All, creates a very large table which will take a while to download and may cause issues with some browsers).
Our Policy on Ordering Clones
MaizeGDB does not distribute maize clones. Read on for further information on clone ordering.
If you are interested in clones that were submitted by Patrick Schnable, you can order clones directly from his lab. Send an email to firstname.lastname@example.org stating the accession number of the clone. There is also a $10.00 charge per clone for handling/preparation. Parties interested in acquiring large sets of clones should contact Dr. Schnable (email@example.com). To learn more about Dr. Schnable's EST distribution policy, click here. To learn more about their EST library information, click here. *A shipping account number(Fed Ex, Airborne, etc.) must be provided if ordering Schnable lab clones so that they can bill the cost of overnight shipping.
If you are interested in clones that were submitted by Virginia Walbot, these clones are available at http://www.genome.arizona.edu/orders/. Be sure to have the GenBank record for the clone ready when you order, as you'll be required to fill in information from the record.
Discussion of EST Data for the General Public
What is an EST?
An EST (or expressed sequence tag) is a short strand of DNA which is part of a cDNA. An EST is usually unique to a particular cDNA, and because cDNAs are pieces of DNA that correspond to a particular gene, ESTs can be used to help identify unknown genes and determine the position of these genes.
Here is an example EST record, p-std707074A08.
What value does storing and studying EST data have?
With ESTs, you can be certain that they have biological significance because of their source. ESTs come from cDNA, which itself is an exact duplication of a piece of DNA that has some biological significance.
What is the connection between corn and EST data?
As with every other organism, EST pieces directly describe important segments of the genetic material of corn. Studying these pieces provides a focus on the pieces of the genome that actually perform important functions.
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Last updated 1:43 pm, Dec 03, 2014.